Cucurbit yellow stunting disorder virus Detected in Pigweed in Florida
نویسندگان
چکیده
منابع مشابه
Geographic distribution and phylogenetic analysis of cucurbit yellow stunting disorder virus in Iran.
Cucurbit yellow stunting disorder virus (CYSDV) is a destructive virus of cucurbits in Iran. During 2008-2012 growing seasons a total of 366 cucurbit samples including melon, cucumber, snakemelon and squash with typical symptoms of CYSDV infection were collected from ten Provinces in Iran. They were screened by ELISA and the infectivity of ELISA-positive samples was confirmed by RT-PCR. The res...
متن کاملGenetic diversity of a natural population of Cucurbit yellow stunting disorder virus.
An analysis of nucleotide sequences in five coding and one non-coding genomic regions of 35 Cucurbit yellow stunting disorder virus (CYSDV) isolates collected on a local scale over an 8 year period is reported here. In total, 2277 nt were sequenced for each isolate, representing about 13 % of the complete virus genome. Mean nucleotide diversity for the whole population in synonymous positions i...
متن کاملIn Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts
Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities...
متن کاملGeographically distant isolates of the crinivirus Cucurbit yellow stunting disorder virus show very low genetic diversity in the coat protein gene.
The population structure and genetic variation of Cucurbit yellow stunting disorder virus (CYSDV) isolates were estimated by single-strand conformation polymorphism and nucleotide sequence analyses of the CYSDV coat protein gene. Analysis of 71 isolates collected from Spain, Jordan, Turkey, Lebanon, Saudi Arabia and North America showed that, from a genetic viewpoint, these isolates could be di...
متن کاملAssessing the Movement of Cucurbit yellow stunting disorder virus in Susceptible and Tolerant Cucumber Germplasms Using Serological and Nucleic Acid-based Methods
Cucurbit yellow stunting disorder virus (CYSDV) is an emerging virus causing significant yield losses in cucurbits. Simple but reliable detection and quantification methods are important tools for disease management. In susceptible germplasm, CYSDV was detected 5 days postinoculation (dpi) by reverse transcriptase polymerase chain reaction (RT-PCR) or by tissue blot immunoassay (TBIA), and 8–9 ...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2011
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-11-10-0813